Kuyini ikhamera Goryaeva? Imithetho kwamangqamuzana egazi ngokubala

Namuhla Goryaeva ikhamera ingenye amadivayisi esetshenziswa kakhulu ukuhlola i-laboratory. Ingasetshenziselwa ukubala inani lamaseli ahlukahlukene igazi. Njengoba yaziwa, ukuhlolwa kwegazi ibaluleke kakhulu inqubo ukuxilongwa kanye nokwelashwa cishe zonke izifo.

Kuyini ikhamera Goryaeva?

Okunjalo owaziwa nedivayisi ewusizo lakhiwe futhi ezihlongozwayo udokotela Russian N. K. Goryaevym. Goryaeva ikhamera, eqinisweni, kuyinto ethize ingilazi slide camera nge anezikhala encanyana.

Kungani ukusebenzisa lolu ikhamera? Ngokuyinhloko-ke kungasiza ukucacisa inombolo kwamangqamuzana egazi, e-leukocyte ethile futhi erythrocyte. Phela, ngosizo yayo kungenzeka ukunquma nesanda microscope esetshenziswa. Le formula for lokhu kanje:

• Kg = (m2 - M1) / i

Kulokhu:

• Kg - nesanda microscope;
• m2 - isikhundla nelungelo umngcele Goryaeva ikhamera cell;
• M1 - isikhundla Umngcele ongakwesokunxele;
• futhi - usayizi kuseli eyodwa, okuyinto ejwayelekile futhi 0.05 mm;
• N - kuyinto inani lamaseli phakathi kulinganiswa ekamelweni imingcele.

Isakhiwo ikhamera

Goryaev ikhamera lutho innoe njengoba slide ingilazi (kodwa mkhulu kakhulu kunokujwayelekile) ehlukaniswa anemifantu transverse izingxenye ezintathu. Ingxenye phakathi ingilazi iqukethe igridi wokusebenza wokubala. Ingxenye yokugcina egumbini efanayo esetshenziselwa yokugcoba coverglass - ngaleyo ndlela, umgcini is wadala ekamelweni avaliwe capillary izikhala ezinhlangothini futhi lapho ligcwele ketshezi.

Ngokuqondene igridi, ekamelweni Goryaeva sehlukaniswe 225 izikwele ezinkulu usayizi alinganayo - ukuthi akuphi imigqa nanhlanu. 25 izikwele ezinkulu zibuye zihlukaniswe ezincane, 16 ngamunye. Ubude ohlangothini Isikwele esincane ngamunye 0.05 mm.

Amalungiselelo ekubalweni izakhi kwakhiwa

Yiqiniso, kulesi simo, kuba inqubo ebalulekile-laboratory luhlolo. Yiqiniso, zonke Goryaeva ekamelweni evele kufanele kuhlanzeke futhi yomile. Ngemva kwalokho, ubhontshisi ugaywe ikhava ingilazi ukuze ungase ubone izici indandatho elikhanyayo. Kusemqoka ukuthi ekamelweni wayengekho air Bubbles, njengoba lokhu kungase kubangele ukuba sisibheke imiphumela ukuhlaziywa.

Njengoba kulindelekile, ukuze ukubala inani corpuscles, reagents ezahlukene asetshenziselwa ezihlukahlukene egazi ngamunye. Ngokwesibonelo, isetshenziselwa ukubala erythrocyte 0.9% isixazululo sodium chloride. Ngaphandle kwesibeletho ukuxubanisa 8 ml kasawoti kanye 0.02 ml igazi. Ngakho, i-laboratory dilutes igazi izikhathi ezingu-400. Ngezinye izikhathi yokuzalanisa zingaba nkulu kakhulu.

Ukuze ubale inani leukocyte, kubalulekile ukuba uhlanganise 0.4 ml acetic acid (ukuthatha 3% noma 5% isixazululo) kanye 0.02 ml igazi.

Ngemva izithako kuxutshwe ngamapayipi ukuhlolwa, usebenzisa pipetting ekhethekile ukuthola inani elincane ingxube kanye negumbi egcwele ngokucophelela i-akhawunti (ngokuvamisile, esisodwa noma ezimbili amathonsi kungaba okwanele).

Kanjani ukubala inani lamaseli igazi?

Ukubala of abomvu egazi ashaye Goryaeva ekamelweni wenziwa izikwele ezinhlanu ezinkulu, okuyinto ilingana ayisishiyagalombili encane. Ukuze ugweme amaphutha ngenxa ukusatshalaliswa engalingani amasampula egazi, khetha izikwele ahlelwe ngokuphambeneyo. ukunakekelwa okukhethekile ikhokhelwa kuze amaseli elise emaphethelweni - ezibhekwa abomvu egazi ashaye ngakwesokunxele bese phezulu kwezindonga, kodwa ungathathi kwalabo abasendleleni phansi futhi imigqa ngqo akhawunti.

Ukuze anqume inani abomvu egazi ashaye ngemililitha legazi ngalinye, inani lamaseli e izikwele ezinhlanu ezinkulu iphindwe 20 000 (uma buhlanjululwe izikhathi 400).

Leukocyte ekamelweni Goryaeva acabange ngokuhlukile. Lapha kubalulekile ukubala inani izakhi okungenani eziyikhulu izikwele ezinkulu. Inombolo okuholela lihlukaniswe by 1600, bese iphindwe 4000, bese 20 (dilution).