Linked immunosorbent Assay: isicelo netimphawu

Linked Assay immunosorbent - cwaningo laboratory, ogxile ukuhlonza izakhiwo "antigen-omzimba" nge amalebula akhethekile enzymatic ezikhomba, ngokushintsha umbala substrate. Injongo eyinhloko yale ukuhlaziywa - ukuzimisela khona imikhiqizo ehlukahlukene ukusabela enzymatic ku amasampula test.

Ukuze ukuthola antigen kanye namasosha omzimba heterogeneous enzyme immunoassay isetshenziswa, kusukela inqubo yayo Kulula ukuhlukanisa izingxenye ukusabela futhi uyephuza ezinye zazo esigabeni okuqinile.

Kuyaphawuleka izinzuzo ezilandelayo kulolu cwaningo laboratory:

• sensitivity high, elikwazi ukuzwa ngisho inombolo encane molecule izinto;

• I-sifeza lesi ukuhlaziywa Ungasebenzisa inombolo okungenani ukuhlolwa impahla;

• kungenzeka isikhathi eside ukugcina zonke izingxenye ezidingekayo ukuze uthole ukuhlaziya;

• Kulula ukwenza;

• ukutholakala Imiphumela wezinsimbi futhi ezibukwayo;

• immunosorbent Assay ngemishini esigabeni ngasinye ukusabela;

• iphansana izindleko kits asetshenziselwa uphethwe.

Lezi izinzuzo ukusebenzisa olubanzi IFA ezindaweni ezihlukahlukene imithi, okubandakanya sitholakele amagciwane.

Inqubo ezivame kakhulu immobilizing amasosha omzimba futhi antigen kuyinto ukusabela adsorption, lapho amangqamuzana ahlukene anamekwe lwesigaba okuqinile (ngenxa ekusebenzisaneni ionic noma hydrophobic noma ukwakheka hydrogen izibopho).

Mangisho ukuthi immunoassay enzyme ibizwa ukusetshenziswa emacaleni amabili:

• ukuhlonza antigen kwamagciwane izifo eziningi;

• ukucacisa khona amasosha omzimba kuya antigen microorganism sokugembula (kufanele kuphawulwe ukuthi ELISA kungathola cishe yinoma iziphi izifo zangasese).

polystyrene ipuleti Special yasetshenziselwa bafeze lo serodiagnosis. It has 96 izimbobo ezinhlangothini antigen adsorbed. Phezu entry ithebhulethi assayed iseli antigen amasosha omzimba inamathelisiwe ezifanele. Ukwengeza okwalandela we omzimba esinelebuli ukuba imithombo immunoglobulin, ayisiza womuntu ubabonisa ukuphendula ethize. In the ukwelashwa kulandela serum chromogen (udayi) kuyahlukahluka Umbala substrate (e nokuba khona izakhiwo elihambisana "antigen-omzimba"). Kuyaphawuleka ukuthi ukushuba umbala ngqo wakhona lemali kwamasosha omzimba.

ELISA Ukuhlaziywa iqede yokulinganisa ukuminyana optical ketshezi e imithombo. zisebenzisa Ngakho amadivayisi akhethekile amaphethini stereotyped ezisiza ukubala lokuhlushwa omzimba. Kufanele kukhunjulwe ukuthi zonke uhlelo test yokwenza lokhu ukuhlaziywa has amagugu ayo izinkomba okujwayelekile (lokhu ngokuvamile ebekiwe imiphumela, okuyinto wakhipha).

Ukusebenzisa ELISA ukuxilonga ugcunsula, isifo sokusha kwesibindi kohlobo ezibangelwa amagciwane kanye negciwane lesandulela ngculaza, kanye ezitholakala emzimbeni cytomegalovirus womuntu, chlamydia, herpes (izinhlobo ezahlukene). Lokhu kuhlaziya futhi uyasiza ekuboneni izinga lama-hormone ukuthola umdlavuza iziphambano.

Kuphi ukuba bahlolelwe eMoscow? Namuhla, kunenqwaba laboratories ezikhethekile, enganikeza anhlobonhlobo ukuhlolwa serological. Ndlela sicela uxhumane nodokotela emtholampilo wendawo (yangasese noma professional, dveryaete). Nge imiphumela yokuhlolwa kufanele ukuya kudokotela. Ulalele imibono yakhe isidingo iyiphi indlela yokwelapha.